Why are hemolyzed samples rejected?

Why are hemolyzed samples rejected?

This is nearly five-times higher than other causes such as clotted samples, inadequate procedures for collection, insufficient volume, and incorrect samples. In vitro hemolysis is the leading cause of specimen rejection for both outpatient and inpatient samples, as well as urgent and routine specimens.

Which lab values are affected if the blood specimen was hemolyzed?

Certain lab tests can be affected and the reported results will be inaccurate. It falsely decreases values such as RBC’s, HCT, and aPTT. It can also falsely elevate potassium, ammonia, magnesium, phosphorus, AST, ALT, LDH and PT.

Why are hemolyzed specimens unacceptable for testing?

Common Causes of Unacceptable Specimens or Inaccurate Test Results: Hemolysis Lysis of red blood cells during collection or handling of blood specimens may cause hemoglobulin or other components to escape from the cells into the serum or plasma.

Does hemolysis affect alkaline phosphatase?

The findings of this study indicate that blood cell hemolysis reduces the activity of ALP which is directly proportional to the level of hemolysis. Released Mg+2 ions were found to inhibit ALP activity in the blood hemolysed samples.

What does it mean when a sample is hemolyzed?

The term hemolysis designates the pathological process of breakdown of red blood cells in blood, which is typically accompanied by varying degrees of red tinge in serum or plasma once the whole blood specimen has been centrifuged.

What substances are increases in a hemolyzed specimen?

Hemolysis may falsely increase the following analytes: AST, alanine transaminase (ALT), LDH, total bilirubin, glucose, calcium, phosphorus, total protein, albumin, magnesium, amylase, lipase, creatine kinase (CK), iron, hemoglobin, and mean corpuscular hemoglobin concentration (MCHC).

What does a hemolyzed specimen mean?

How do you prevent a hemolyzed specimen?

Best Practices to Prevent Hemolysis

  1. Use the correct needle size for blood collection (20-22 gauge).
  2. Avoid using butterfly needles, unless specifically requested by patient.
  3. Warm up the venipuncture site to increase blood flow.
  4. Allow disinfectant on venipuncture site to dry completely.

What does it mean if blood is hemolyzed?

What happens when blood Hemolyzed?

“Hemo” means blood, of course; “lysis” means to rupture or the destruction of cells. So hemolysis is literally the destruction of blood cells, specifically red blood cells. When red cells rupture, they spill their contents, mostly hemoglobin, into their surroundings.

Why do specimens get hemolyzed?

Hemolysis may be due to specimen collection, processing, or transport. Hemolysis may also be due to pathological conditions, such as immune reactions, infections, medications, toxins and poisons, and therapeutic procedures. Frequently, laboratory testing can still be performed on a hemolyzed sample.

Why do blood banks reject hemolyzed blood specimens?

Abstract Background: Blood banks have historically rejected hemolyzed specimens for ABO type and antibody screen based on concerns of artifactual interference with test performance or the detection of incompatibility. Samples from emergency departments (EDs) are commonly discarded due to hemolysis or mislabeling.

Why are hemolysis samples unsuitable for routine testing?

Hemolytic samples are a rather common and unfavorable occurrence in laboratory practice, as they are often considered unsuitable for routine testing due to biological and analytical interference. However, definitive indications on the analytical and clinical management of hemolyzed specimens are currently lacking.

Is it safe to discard hemolyzed blood specimens?

Our data, although device-specific, are adequate to suggest that sample discard and recollection due to hemolysis in blood bank specimens should be reconsidered with potential benefits for patient safety and efficiency. Publication types Evaluation Study Validation Study

Are there false positive antibody screens after hemolysis?

Twenty of 31 positive antibody screens became uninterpretable after experimental hemolysis without any threshold supernatant Hb being evident, likely explained by the duration of specimen storage before experimental hemolysis. No false-positive or false-negative samples were observed in the antibody screen of the hemolyzed specimens.

Abstract Background: Blood banks have historically rejected hemolyzed specimens for ABO type and antibody screen based on concerns of artifactual interference with test performance or the detection of incompatibility. Samples from emergency departments (EDs) are commonly discarded due to hemolysis or mislabeling.

Why is sample hemolysis a problem in emergency departments?

Causes, consequences and management of sample hemolysis in the clinical laboratory Preanalytical hemolysis of blood samples is a common problem in medical practice, especially in emergency departments. Several potential influences on sample hemolysis have been investigated, including sampling techniques, centrifugation and sample transport.

What’s the reaction to a hemolyzed blood test?

The most common reaction to a hemolyzed blood test result is to take another blood sample. A different location may be chosen and the technician taking the sample may try using a different needle gauge to lessen the risks of a second occurrence.

Our data, although device-specific, are adequate to suggest that sample discard and recollection due to hemolysis in blood bank specimens should be reconsidered with potential benefits for patient safety and efficiency. Publication types Evaluation Study Validation Study