What are the clinical significance of determination of cholesterol from blood?
A complete cholesterol test is done to determine whether your cholesterol is high and to estimate your risk of heart attacks and other forms of heart disease and diseases of the blood vessels. A complete cholesterol test includes the calculation of four types of fats in your blood: Total cholesterol.
What determines your blood cholesterol level?
Saturated fat and cholesterol in the food you eat make your blood cholesterol level rise. Saturated fat is the main problem, but cholesterol in foods also matters. Reducing the amount of saturated fat in your diet helps lower your blood cholesterol level.
What method is used for analysis of cholesterol?
Multiple analytical methods have been developed for analysis of cholesterol, including classical chemical methods, enzymatic assays, gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS).
What is the most common method for cholesterol?
The enzymatic method is the principal method used for measuring cholesterol levels in plasma, where cholesterol is solubilized in an aqueous solution through its incorporation in lipoproteins.
How is lipid profile estimation of cholesterol done?
Experiment 5a Lipid profile Estimation of Cholesterol in serum by enzymatic method Experiment 5a Lipid profile Estimation of Cholesterol in serum by enzymatic method Background: Cholesterol is a steroid with a secondary hydroxyl group in the C position and found in blood, bile and brain tissue.
How is cholesterol measured in a blood test?
SUMMARY OF TEST PRINCIPLES AND CLINICAL RELEVANCE A. Total Cholesterol Cholesterol is measured enzymatically in serum or plasma in a series of coupled reactions that hydrolyze cholesteryl esters and oxidize the 3-OH group of cholesterol. One of the reaction byproducts, H 2O
How to calculate the dilution factor for cholesterol?
Calculation: Serum total cholesterol (mg/dL) = (T-B)/ (S-B) x Concentration of Standard x Dilution factor Serum total cholesterol (mg/dL) = (T-B)/ (S-B) x 200 mg/dL x Dilution factor Dilution factor = (0.1/8)/ (0.05/8) = 2 38. Protocol for HDL-Cholesterol Estimation
How are triglycerides and lipoproteins measured in blood?
Analysis of plasma or serum total cholesterol, triglycerides, and lipoproteins is usually performed on blood specimens obtained by venipuncture in tubes containing EDTA. Because of a larger content of water, plasma levels have been found to be around 3% lower than matched serum levels.